Source: Hindu
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Focus Area |
Feature |
Conservation and Health Initiatives |
●Preserve indigenous cattle breeds using NGS. ●New-generation vaccines against livestock diseases (e.g., brucellosis). |
Industry Collaboration and Innovation |
●Boost livestock economy through food security, vaccines, and diagnostics. ●Develop bio-scaffolds and a 3D model for TB research. |
R&D |
●Identify TB resistance in cattle; explore CRISPR for productivity. ●Promote sustainable protein and bacteriophage alternatives. ●NIAB’s R&D efforts align with the BioE3 policy to boost bio-manufacturing and position India as a global leader in biotechnology. |
Diagnostics and Sustainable Farming |
●Develop kits for brucellosis, mastitis, and hormone detection. ●MILAN (Meeting of Livestock Farmers) showcases sustainable farming. ●Use aquatic weed and yeast protein to cut emissions. |
Alternative Nutrition and Feed |
●Aquatic Weed: Being introduced as potential livestock feed. ●Yeast-Derived Protein: Substitution in regular feed formulations to boost productivity. |
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The DNA fragments in the library need to be multiplied to ensure a strong enough signal during sequencing.
All DNA fragments are sequenced at the same time using an NGS machine.
Read basics of Genome Sequencing: https://www.iasgyan.in/daily-current-affairs/10000-genome-project-completed#:~:text=Importance%3A%20Genome%20sequencing%20enables%20scientists,diseases%2C%20and%20develop%20personalized%20treatments.
Read about DNA: https://www.iasgyan.in/daily-current-affairs/human-genome-project
Category |
Whole Genome Sequencing (WGS) |
Targeted Sequencing: Exome Sequencing |
Targeted Sequencing: Gene or Region-Specific Panels |
|
Description |
Sequencing the entire genome |
Sequencing only exons (protein-coding regions) |
Sequencing regions of interest such as disease-associated genes or genomic hotspots |
|
Pros |
●Most comprehensive genome coverage ●Detects widest range of features: indels, structural and copy number variants, regulatory elements ●No bias from PCR amplification or probe hybridization ●Best for discovery research |
●1% of human genome, less data to analyze than WGS ●Faster workflow than WGS ●Multiplexing small number of samples ●Medium sample input (50 ng–1 μg depending on library prep method) |
● Highly flexible, customizable designs ●Data is focused specifically on regions/genes of interest ●Lowest sample input (10 ng) ●Multiplexing large numbers of samples ●Better for detecting rare alleles |
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Cons |
●Generates a lot of potentially unnecessary data from non-coding/non-functional regions ●Data is very complicated ●Multiplexing usually not possible |
●Only provides data on exons (may miss functionally relevant variants) ●May include extra data not needed for small gene studies |
●Only provides data on targeted regions (may miss relevant variants if not in design) |
|
Speed/Return of Results |
Slowest |
Medium |
Fastest |
|
Cost |
$$$ |
$$ |
$ |
|
Data Volume |
Largest |
Medium |
Smallest |
|
When to Use |
●Complete coverage of genome needed ●De novo assembly ●Discovery of unknown genomic variants causing a disease ●Aneuploidy detection (preimplantation genetic testing) |
●Disease-specific research projects ●Clinical sequencing
|
●Clinical sequencing ●Disease-specific research projects ●Inherited disease ●Oncology ●Immune repertoire ●Liquid biopsy |
RNA type |
Function |
Messenger RNA |
Codes for protein |
Ribosomal RNA |
Translation |
Transfer RNA |
Translation |
Small nuclear RNA |
Splicing and other functions |
Small nucleolar RNA |
Nucleotide modification of RNAs |
Small Cajal body-specific RNA |
Type of snoRNA; nucleotide modification of RNAs |
Long noncoding RNA |
Regulation of gene transcription; epigenetic regulation |
MicroRNA |
Gene regulation |
Piwi-interacting RNA |
Transposon defense |
Small interfering RNA |
Gene regulation |
RNA sequencing method |
Description and benefits |
Total RNA |
●Whole transcriptome analysis to examine coding and noncoding RNA simultaneously; suitable for novel discovery. ●More throughput intensive to achieve high enough coverage for discovery. ●Potential inefficiencies and bias due to different sequencing lengths. |
mRNA sequencing |
●Able to identify novel and known content |
smRNA sequencing |
●Isolation of small RNA to focus study on noncoding RNA to identify novel and known content such as microRNA (miRNA) |
Targeted RNA sequencing |
●Sequencing specific transcripts of interest to focus efforts and lower cost to analyze specific genes of interest. |
Sources:
PRACTICE QUESTION Q: Consider the following statements regarding types of RNA:
Which of the above statements is/are correct? a) 1 only
Answer: c) Explanation: 1st statement is correct: PIWI-interacting RNAs (piRNAs) of 21–35 nucleotides in length silence transposable elements, regulate gene expression and fight viral infection. 2nd statement is correct: microRNA is the name of a family of molecules that helps cells control the kinds and amounts of proteins they make. That is, cells use microRNA to help control gene expression. Molecules of microRNA are found in cells and in the bloodstream. |
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